Catalog No.
YHK78901
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Ser914-Lys1160
Predicted molecular weight
29.78 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9UHB7
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Major CDK9 elongation factor-associated protein, MCEF, Protein AF-5q31, ALL1-fused gene from chromosome 5q31 protein, AF5Q31, AFF4, AF4/FMR2 family member 4
A common molecular mechanism underlying Cornelia de Lange and CHOPS syndromes., PMID:39983729
FENDRR represses Bladder Cancer Cell Proliferation, Stemness, Migration, Invasion, and EMT Process by Targeting miR-18a-5p/AFF4 Axis., PMID:39572480
N6-methyladenosine-modified circCDK14 promotes ossification of the ligamentum flavum via epigenetic modulation by targeting AFF4., PMID:39414635
MeCP2 Interacts with the Super Elongation Complex to Regulate Transcription., PMID:39005382
Multiple Genomic Alterations, Including a Novel AFF4::IRF1 Fusion Gene, in a Treatment-Refractory Blastic Plasmacytoid Dendritic-Cell Neoplasm: A Case Report and Literature Review., PMID:38203475
Impact of disulfidptosis-associated clusters on breast cancer survival rates and guiding personalized treatment., PMID:38116315
PRMT2 promotes HIV-1 latency by preventing nucleolar exit and phase separation of Tat into the Super Elongation Complex., PMID:37949879
The Biological Significance of AFF4: Promoting Transcription Elongation, Osteogenic Differentiation and Tumor Progression., PMID:37815186
AFF4 regulates osteogenic potential of human periodontal ligament stem cells via mTOR-ULK1-autophagy axis., PMID:37731335
AFF4 globally affects the release of paused RNA polymerase II in HEL cells., PMID:37609817
Distinct roles of two SEC scaffold proteins, AFF1 and AFF4, in regulating RNA polymerase II transcription elongation., PMID:37528066
Genomic analyses in Cornelia de Lange Syndrome and related diagnoses: Novel candidate genes, genotype-phenotype correlations and common mechanisms., PMID:37377026
[AF4/FMR2 and IL-10 gene single nucleotide polymorphisms are correlated with disease susceptibility and immune infiltration in ankylosing spondylitis]., PMID:37313815
PI3K/ c-Myc/AFF4 axis promotes pancreatic tumorigenesis through fueling nucleotide metabolism., PMID:37063434
The super elongation complex drives transcriptional addiction in MYCN-amplified neuroblastoma., PMID:36989355
MAPK Is a Mutual Pathway Targeted by Anxiety-Related miRNAs, and E2F5 Is a Putative Target for Anxiolytic miRNAs., PMID:36979479
Unexpected appearance of KMT2A::MLLT10 fusion transcript in acute myeloid leukemia with t(5;11)(q31;q23.3)., PMID:36774707
Analysis of serum circulating MicroRNAs level in Malaysian patients with gestational diabetes mellitus., PMID:36434110
CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway., PMID:36401176
AFF4 regulates cellular adipogenic differentiation via targeting autophagy., PMID:36149892
CDK9 activity switch associated with AFF1 and HEXIM1 controls differentiation initiation from epidermal progenitors., PMID:35906225
A transcriptionally repressed quiescence program is associated with paused RNA polymerase II and is poised for cell cycle re-entry., PMID:35781573
Circular RNA hsa_circ_0057452 facilitates keloid progression by targeting the microRNA-1225-3p/AF4/FMR2 family member 4 axis., PMID:35706403
A proteolysis-targeting chimera molecule selectively degrades ENL and inhibits malignant gene expression and tumor growth., PMID:35395864
Disrupting the Cdk9/Cyclin T1 heterodimer of 7SK snRNP for the Brd4 and AFF1/4 guided reconstitution of active P-TEFb., PMID:34935961
MiR-425-5p accelerated the proliferation, migration, and invasion of ovarian cancer cells via targeting AFF4., PMID:34686190
The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells., PMID:34658793
BETting on a Transcriptional Deficit as the Main Cause for Cornelia de Lange Syndrome., PMID:34386522
Small-molecule inhibitor of AF9/ENL-DOT1L/AF4/AFF4 interactions suppresses malignant gene expression and tumor growth., PMID:34373735
Circular RNA AFF4 modulates osteogenic differentiation in BM-MSCs by activating SMAD1/5 pathway through miR-135a-5p/FNDC5/Irisin axis., PMID:34145212
Variants in the degron of AFF3 are associated with intellectual disability, mesomelic dysplasia, horseshoe kidney, and epileptic encephalopathy., PMID:33961779
Identification of the transcription factor, AFF4, as a new target of miR-203 in CNS., PMID:33878354
Flash forward genetics: new twists in transcription across evolutionary boundaries., PMID:33554445
Therapeutic targeting of transcriptional elongation in diffuse intrinsic pontine glioma., PMID:33471107
Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression., PMID:33446572
AFF4 facilitates melanoma cell progression by regulating c-Jun activity., PMID:33417923
Modeling by disruption and a selected-for partner for the nude locus., PMID:33369874
CRISPRi-mediated depletion of Spt4 and Spt5 reveals a role for DSIF in the control of HIV latency., PMID:33333262
A case of CHOPS syndrome accompanied with moyamoya disease and systemic vasculopathy., PMID:33248856
AFF4 regulates osteogenic differentiation of human dental follicle cells., PMID:32606293
Inhibition of the Super Elongation Complex Suppresses Herpes Simplex Virus Immediate Early Gene Expression, Lytic Infection, and Reactivation from Latency., PMID:32518191
ENL initiates multivalent phase separation of the super elongation complex (SEC) in controlling rapid transcriptional activation., PMID:32270036
Super Elongation Complex as a Targetable Dependency in Diffuse Midline Glioma., PMID:32268092
Acetylation of histone H3K27 signals the transcriptional elongation for estrogen receptor alpha., PMID:32265480
AFF4 enhances odontogenic differentiation of human dental pulp cells., PMID:32139123
Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription., PMID:32128251
CircRNA AFF4 promotes osteoblast cells proliferation and inhibits apoptosis via the Mir-7223-5p/PIK3R1 axis., PMID:31848327
Long noncoding RNA ZFPM2-AS1 is involved in lung adenocarcinoma via miR-511-3p/AFF4 pathway., PMID:31692047
Genetic Disorders in Prenatal Onset Syndromic Short Stature Identified by Exome Sequencing., PMID:31630891
The bioinformatics aspects of gene screening of HT-29, human colon cell line treated with caffeic acid., PMID:31528309
For research use only. Not for human or drug use.
