Catalog No.
YHK76401
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Lys407-His689
Predicted molecular weight
34.68 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q7L2J0
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
MEPCE, Bin3 homolog, Bicoid-interacting protein 3 homolog, 7SK snRNA methylphosphate capping enzyme, MePCE, BCDIN3
MePCE promotes homologous recombination through coordinating R-loop resolution at DNA double-stranded breaks., PMID:40411785
An integrative systems-biology approach defines mechanisms of Alzheimer's disease neurodegeneration., PMID:40393985
The phosphatase PP1 sustains global transcription by promoting RNA polymerase II pause release., PMID:39603240
LARP3, LARP7, and MePCE are involved in the early stage of human telomerase RNA biogenesis., PMID:39009594
Comparative Analysis of Two NNMT Bisubstrate Inhibitors through Chemoproteomic Studies: Uncovering the Role of Unconventional SAM Analogue Moiety for Improved Selectivity., PMID:38181447
Drosophila Amus and Bin3 methylases functionally replace mammalian MePCE for capping and the stabilization of U6 and 7SK snRNAs., PMID:38100593
Catalytic activity of the Bin3/MePCE methyltransferase domain is dispensable for 7SK snRNP function in Drosophila melanogaster., PMID:37982586
The fission yeast methyl phosphate capping enzyme Bmc1 guides 2'-O-methylation of the U6 snRNA., PMID:37403782
Excessive transcription-replication conflicts are a vulnerability of BRCA1-mutant cancers., PMID:36928661
Multiple RNA-binding proteins associated with long interspersed element-1 encoded ORF1p are targeted by the autoimmune response in systemic lupus erythematosus., PMID:39606792
Elevated expression of LIF predicts a poor prognosis and promotes cell migration and invasion of clear cell renal cell carcinoma., PMID:35992780
Structural basis of RNA conformational switching in the transcriptional regulator 7SK RNP., PMID:35320752
The methyl phosphate capping enzyme Bmc1/Bin3 is a stable component of the fission yeast telomerase holoenzyme., PMID:35277511
A putative cap binding protein and the methyl phosphate capping enzyme Bin3/MePCE function in telomerase biogenesis., PMID:35217638
Tip110 Expression Facilitates the Release of HEXIM1 and pTEFb from the 7SK Ribonucleoprotein Complex Involving Regulation of the Intracellular Redox Level., PMID:34881089
Interaction of 7SK with the Smn complex modulates snRNP production., PMID:33627647
Long non‑coding RNA ST8SIA6‑AS1 promotes the migration and invasion of hypoxia‑treated hepatocellular carcinoma cells through the miR‑338/MEPCE axis., PMID:33416148
Stabilize and connect: the role of LARP7 in nuclear non-coding RNA metabolism., PMID:32401147
JMJD6 cleaves MePCE to release positive transcription elongation factor b (P-TEFb) in higher eukaryotes., PMID:32048991
Crystal structure of human cytoplasmic tRNAHis-specific 5'-monomethylphosphate capping enzyme., PMID:31919512
de novo MEPCE nonsense variant associated with a neurodevelopmental disorder causes disintegration of 7SK snRNP and enhanced RNA polymerase II activation., PMID:31467394
Structural basis of 7SK RNA 5'-γ-phosphate methylation and retention by MePCE., PMID:30559425
CDK9: a signaling hub for transcriptional control., PMID:30227759
Crosstalk between the RNA Methylation and Histone-Binding Activities of MePCE Regulates P-TEFb Activation on Chromatin., PMID:29425494
Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs., PMID:29051200
In silico identification of potential key regulatory factors in smoking-induced lung cancer., PMID:28592245
Reconstitution of a functional 7SK snRNP., PMID:28431135
The 7SK snRNP associates with the little elongation complex to promote snRNA gene expression., PMID:28254838
The crystal structure of the 5΄ functional domain of the transcription riboregulator 7SK., PMID:28082395
Solution structure of the 5'-terminal hairpin of the 7SK small nuclear RNA., PMID:27852926
7SK small nuclear RNA transcription level down-regulates in human tumors and stem cells., PMID:27752877
SAINTq: Scoring protein-protein interactions in affinity purification - mass spectrometry experiments with fragment or peptide intensity data., PMID:27119218
Genome-wide analysis of KAP1, the 7SK snRNP complex, and RNA polymerase II., PMID:26981421
Intermolecular recognition of the non-coding RNA 7SK and HEXIM protein in perspective., PMID:25863285
Release of positive transcription elongation factor b (P-TEFb) from 7SK small nuclear ribonucleoprotein (snRNP) activates hexamethylene bisacetamide-inducible protein (HEXIM1) transcription., PMID:24515107
Calpain 2 activation of P-TEFb drives megakaryocyte morphogenesis and is disrupted by leukemogenic GATA1 mutation., PMID:24369834
RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP., PMID:23471002
Human RNA methyltransferase BCDIN3D regulates microRNA processing., PMID:23063121
The Bin3 RNA methyltransferase targets 7SK RNA to control transcription and translation., PMID:22740346
Ars2 promotes proper replication-dependent histone mRNA 3' end formation., PMID:22244333
7SK snRNA: a noncoding RNA that plays a major role in regulating eukaryotic transcription., PMID:21853533
Implication of genetic variants near TMEM18, BCDIN3D/FAIM2, and MC4R with coronary artery disease and obesity in Chinese: a angiography-based study., PMID:21625854
A capping-independent function of MePCE in stabilizing 7SK snRNA and facilitating the assembly of 7SK snRNP., PMID:19906723
LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated., PMID:18281698
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme., PMID:17643375
For research use only. Not for human or drug use.
