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Recombinant Rubella virus/RUBV Polyprotein Protein, N-His

Catalog #:   YVV22801 Specific References (44) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: E. coli
Accession: Q8QL55
Protein length: Asn127-Arg277
Overview

Catalog No.

YVV22801

Expression system

E. coli

Species

Rubella virus (RUBV)

Protein length

Asn127-Arg277

Predicted molecular weight

19.32 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

Q8QL55

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Polyprotein

Data Image
  • SDS-PAGE
    SDS PAGE for recombinant Rubella virus/RUBV Polyprotein Protein
References

Identification of a potential anti-viral drug targeting allosteric site of papain-like protease against rubella using a molecular modeling approach., PMID:39714197

Crystal structure and biochemical activity of the macrodomain from rubella virus p150., PMID:38289106

Crystal structure of the Rubella virus protease reveals a unique papain-like protease fold., PMID:35835220

Immunotherapy with DNA vaccine and live attenuated rubella/SIV gag vectors plus early ART can prevent SIVmac251 viral rebound in acutely infected rhesus macaques., PMID:32130229

Heat Shock Protein 90 Ensures the Integrity of Rubella Virus p150 Protein and Supports Viral Replication., PMID:31484751

Expression of complete SIV p27 Gag and HIV gp120 engineered outer domains targeted by broadly neutralizing antibodies in live rubella vectors., PMID:28483193

Ancient recombination events and the origins of hepatitis E virus., PMID:27733122

The hepatitis E virus ORF1 'X-domain' residues form a putative macrodomain protein/Appr-1″-pase catalytic-site, critical for viral RNA replication., PMID:25870943

Recombinant rubella vectors elicit SIV Gag-specific T cell responses with cytotoxic potential in rhesus macaques., PMID:25802183

Molecular characterization of hepatitis E virus ORF1 gene supports a papain-like cysteine protease (PCP)-domain activity., PMID:23978667

Enhanced expression of HIV and SIV vaccine antigens in the structural gene region of live attenuated rubella viral vectors and their incorporation into virions., PMID:23474312

Live attenuated rubella viral vectors stably express HIV and SIV vaccine antigens while reaching high titers., PMID:22776214

Determinants in the maturation of rubella virus p200 replicase polyprotein precursor., PMID:22491463

Class II enveloped viruses., PMID:21790946

Calcium-dependent association of calmodulin with the rubella virus nonstructural protease domain., PMID:20086014

A cysteine-rich metal-binding domain from rubella virus non-structural protein is essential for viral protease activity and virus replication., PMID:18795894

The rubella virus nonstructural protease recognizes itself via an internal sequence present upstream of the cleavage site for trans-activity., PMID:16570206

A system of protein target sequences for anti-RNA-viral chemotherapy by a vitamin B6-derived zinc-chelating trioxa-adamantane-triol., PMID:14527557

Expression of a recombinant gag protein from endogenous avian virus and its use in screening for antibody reactivity in recipients of chick-derived vaccines., PMID:12922106

Rescue of rubella virus replication-defective mutants using vaccinia virus recombinant expressing rubella virus nonstructural proteins., PMID:12076835

Mutations in the GDD motif of rubella virus putative RNA-dependent RNA polymerase affect virus replication., PMID:11437666

Rubella virus E2 signal peptide is required for perinuclear localization of capsid protein and virus assembly., PMID:11160697

Rubella virus nonstructural protein protease domains involved in trans- and cis-cleavage activities., PMID:10823845

Mutational analysis of the rubella virus nonstructural polyprotein and its cleavage products in virus replication and RNA synthesis., PMID:10799588

Intracellular distribution of rubella virus nonstructural protein P150., PMID:10438871

Proteolytic processing of rubella virus nonstructural proteins., PMID:9656995

Serum antibodies to HIV-1 are produced post-measles virus infection: evidence for cross-reactivity with HLA., PMID:9486389

Characterization of the rubella virus nonstructural protease domain and its cleavage site., PMID:8676497

Identification of an RNA-stimulated NTPase in the predicted helicase sequence of the Rubella virus nonstructural polyprotein., PMID:8599224

Synthesis and processing of the rubella virus p110 polyprotein precursor in baculovirus-infected Spodoptera frugiperda cells., PMID:7754676

Mutational analysis of the arginine residues in the E2-E1 junction region on the proteolytic processing of the polyprotein precursor of rubella virus., PMID:8178466

Expression of recombinant E2 and C proteins of rubella virus in insect cells., PMID:7979996

Computer-assisted assignment of functional domains in the nonstructural polyprotein of hepatitis E virus: delineation of an additional group of positive-strand RNA plant and animal viruses., PMID:1518855

Diagnostic potential of baculovirus-expressed rubella virus envelope proteins., PMID:1774311

Putative papain-related thiol proteases of positive-strand RNA viruses. Identification of rubi- and aphthovirus proteases and delineation of a novel conserved domain associated with proteases of rubi-, alpha- and coronaviruses., PMID:1652473

The influence of capsid protein cleavage on the processing of E2 and E1 glycoproteins of rubella virus., PMID:2053296

Safety and immunogenicity of multiple conventional immunizations administered during early HIV infection., PMID:1675680

Translocation and cleavage of rubella virus envelope glycoproteins: identification and role of the E2 signal sequence., PMID:2273395

The E2 signal sequence of rubella virus remains part of the capsid protein and confers membrane association in vitro., PMID:2214022

In vitro and in vivo expression of rubella virus glycoprotein E2: the signal peptide is contained in the C-terminal region of capsid protein., PMID:2683361

Baculovirus polyhedrin promoter-directed expression of rubella virus envelope glycoproteins, E1 and E2, in Spodoptera frugiperda cells., PMID:2672567

Translocation of rubella virus glycoprotein E1 into the endoplasmic reticulum., PMID:2845137

Sequence of the region coding for virion proteins C and E2 and the carboxy terminus of the nonstructural proteins of rubella virus: comparison with alphaviruses., PMID:2836271

Nucleotide sequence and in vitro expression of rubella virus 24S subgenomic messenger RNA encoding the structural proteins E1, E2 and C., PMID:3562245

Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

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For research use only. Not for human or drug use.

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Recombinant Rubella virus/RUBV Polyprotein Protein, N-His [YVV22801]
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