Catalog No.
YHK69401
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Asp906-Gly975
Predicted molecular weight
21.91 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q5VTR2
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
RING-type E3 ubiquitin transferase BRE1A, E3 ubiquitin-protein ligase BRE1A, BRE1-A, BRE1A, RNF20, hBRE1, RING finger protein 20
RNF20 links the DNA damage response and metabolic rewiring in lung cancer through HIF1α., PMID:40436847
Increasing H2B Monoubiquitination Improves the Transcriptome and Memory in the Aged Hippocampus., PMID:40194842
RNF20 dual regulation of MDA5 signaling to maintain immune homeostasis in chickens., PMID:39998124
Impaired inducibility of immune regulatory capacity of peripheral B cells of patients with recurrent pregnancy loss., PMID:39495420
RNF20-mediated transcriptional pausing and VEGFA splicing orchestrate vessel growth., PMID:39322771
Decoding temporal thermogenesis: coregulator selectivity and transcriptional control in brown and beige adipocytes., PMID:39155481
USP44 Overexpression Drives a MYC-Like Gene Expression Program in Neuroblastoma through Epigenetic Reprogramming., PMID:38775808
RNF20 Reduces Cell Proliferation and Warburg Effect by Promoting NLRP3 Ubiquitination in Liver Cancer., PMID:38608146
Monoubiquitination of histone H2B is a crucial regulator of the transcriptome during memory formation., PMID:38580378
Structure of the human Bre1 complex bound to the nucleosome., PMID:38519511
Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate histone ubiquitination and transcriptional activation of target genes., PMID:38517892
Systems genetics analysis reveals the common genetic basis for pain sensitivity and cognitive function., PMID:38421132
Psychological stress to ovalbumin peptide-specific T-cell receptor transgenic mice impairs the suppressive ability of type 1 regulatory T cell., PMID:38366844
RNF20 contributes to epigenetic immunosuppression through CDK9-dependent LSD1 stabilization., PMID:38315842
Ubiquitin ligase RNF20 coordinates sequential adipose thermogenesis with brown and beige fat-specific substrates., PMID:38296968
Albendazole inhibits colon cancer progression and therapy resistance by targeting ubiquitin ligase RNF20., PMID:38278978
Endoplasmic reticulum stress interferes with the development of type 1 regulating T cells., PMID:38265686
RNF20 Regulates Oocyte Meiotic Spindle Assembly by Recruiting TPM3 to Centromeres and Spindle Poles., PMID:38240347
Impact of Ring Finger Protein 20 and Its Downstream Regulation on Renal Tubular Injury in a Unilateral Nephrectomy Mouse Model Fed a High-Fat Diet., PMID:38068817
The H2Bub1-deposition complex is required for human and mouse cardiogenesis., PMID:38038666
Iron deficiency-induced ferritinophagy impairs skeletal muscle regeneration through RNF20-mediated H2Bub1 modification., PMID:37976359
Epigenetic regulator RNF20 underlies temporal hierarchy of gene expression to regulate postnatal cardiomyocyte polarization., PMID:37967007
KSHV RTA utilizes the host E3 ubiquitin ligase complex RNF20/40 to drive lytic reactivation., PMID:37888983
Pancreatic Cancer-Derived Exosomal miR-Let-7b-5p Stimulates Insulin Resistance in Skeletal Muscle Cells Through RNF20/STAT3/FOXO1 Axis Regulation., PMID:37842335
Requirement of WDR70 for POLE3-mediated DNA double-strand breaks repair., PMID:37682991
Mechanistic insights into nucleosomal H2B monoubiquitylation mediated by yeast Bre1-Rad6 and its human homolog RNF20/RNF40-hRAD6A., PMID:37633270
Adipocyte Rnf20 ablation increases the fast-twitch fibers of skeletal muscle via lysophosphatidylcholine 16:0., PMID:37555936
Cortisol synergizing with endoplasmic reticulum stress induces regulatory T-cell dysfunction., PMID:37475539
RNF20/RNF40 ameliorates streptozotocin-induced type 1 diabetes by activating vitamin D receptors in vivo., PMID:37422774
Maintaining chromosome and genome stability by the RPA-RNF20-SNF2H cascade., PMID:37276422
Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases., PMID:37230987
Endoplasmic reticulum stress impairs the immune regulation property of macrophages in asthmatic patients., PMID:37172666
The RPA-RNF20-SNF2H cascade promotes proper chromosome segregation and homologous recombination repair., PMID:37155876
Wee1 epigenetically modulates H2B mono-ubiquitination at K120 lysine and DNA double-strand break repair through phosphorylation of H2BY37-dependent manner in small-cell lung cancer., PMID:37102239
ER stress modulates the immune regulatory ability in gut M2 cells of patients with ulcerative colitis., PMID:37091242
Antinuclear antibodies in individuals with COVID-19 reflect underlying disease: Identification of new autoantibodies in systemic sclerosis (CDK9) and malignancy (RNF20, RCC1, TRIP13)., PMID:36738952
RNF20 deletion causes inflammation in model of sepsis through the NLRP3 activation., PMID:36650938
Rnf20 inhibition enhances immunotherapy by improving regulatory T cell generation., PMID:36371755
A siRNA screening of UBE2 family demonstrated that UBE2R1 had a high repressive effect on HIV Tat protein., PMID:36275929
The roles of the tumor suppressor parafibromin in cancer., PMID:36211470
Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development., PMID:36103829
DNA Damage Induction Alters the Expression of Ubiquitin and SUMO Regulators in Preimplantation Stage Pig Embryos., PMID:36077022
Histone Mono-Ubiquitination in Transcriptional Regulation and Its Mark on Life: Emerging Roles in Tissue Development and Disease., PMID:35954248
Epoxymicheliolide directly targets histone H2B to inhibit neuroinflammation via recruiting E3 ligase RNF20., PMID:35074526
Comprehensive Atlas of the Myelin Basic Protein Interaction Landscape., PMID:34827627
Molecular characterization of substrate-induced ubiquitin transfer by UBR7-PHD finger, a newly identified histone H2BK120 ubiquitin ligase., PMID:34739193
RNF20 affects porcine adipocyte differentiation via regulation of mitotic clonal expansion., PMID:34647359
Protease cleavage of RNF20 facilitates coronavirus replication via stabilization of SREBP1., PMID:34452991
Massively parallel in vivo CRISPR screening identifies RNF20/40 as epigenetic regulators of cardiomyocyte maturation., PMID:34290256
Histone H2B ubiquitination mediated chromatin relaxation is essential for the induction of somatic cell reprogramming., PMID:34155716
For research use only. Not for human or drug use.
