Catalog No.
YHK69001
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Thr2071-Ala2335
Predicted molecular weight
32.80 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q6P2Q9
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
220 kDa U5 snRNP-specific protein, p220, PRPF8, Splicing factor Prp8, PRP8 homolog, Pre-mRNA-processing-splicing factor 8, PRPC8
The RNA splicing factor PRPF8 is required for left-right organiser cilia function and determination of cardiac left-right asymmetry via regulation of Arl13b splicing., PMID:40501629
Deep clinical and genetic analysis of 17p13.3 region: 38 pediatric patients diagnosed using next-generation sequencing and literature review., PMID:40390087
Discovery of novel ancestry specific genes for androgens and hypogonadism in Million Veteran Program Men., PMID:40316537
Addressing the tissue specificity of U5 snRNP spliceosomopathies., PMID:40264708
Excision repair cross complementation group 1 gene exon 3 skipping isoform presents selective cGAS-STING activation in platinum-sensitive lung adenocarcinoma., PMID:40258523
Putrescine modulates alternative splicing of gonadotropin hormone-releasing hormone (GnRH) through differential regulation of splicing factors., PMID:40239543
Research Progress on the Relationship Between PRPF8 and Cancer., PMID:40136404
Reporting a Homozygous Case of Neurodevelopmental Disorder Associated With a Novel PRPF8 Variant., PMID:40066647
Retinitis pigmentosa-linked mutations impair the snRNA unwinding activity of SNRNP200 and reduce pre-mRNA binding of PRPF8., PMID:40045025
NLRP7 maintains the genomic stability during early human embryogenesis via mediating alternative splicing., PMID:39865169
De novo and inherited dominant variants in U4 and U6 snRNAs cause retinitis pigmentosa., PMID:39830270
The splicing machinery is dysregulated and represents a therapeutic vulnerability in breast cancer., PMID:39725737
Identification of SNHG11 as a Therapeutic Target in Pulmonary Hypertension., PMID:39265177
LncRNA ZNF649-AS1 promotes trastuzumab resistance and TAM-dependent PD-L1 expression in breast cancer by regulating EXOC7 alternative splicing., PMID:39159899
[Application of whole exome sequencing technology in the diagnosis of hereditary eye diseases]., PMID:38978368
A de novo PRPF8 Pathogenic Variant in Transient Severe Hypophosphatemia with Delayed Puberty and Growth Failure., PMID:38976971
Spliceosomic dysregulation in pancreatic cancer uncovers splicing factors PRPF8 and RBMX as novel candidate actionable targets., PMID:38790138
Biological relevance of alternative splicing in hematologic malignancies., PMID:38760666
PRPF8-mediated dysregulation of hBrr2 helicase disrupts human spliceosome kinetics and 5´-splice-site selection causing tissue-specific defects., PMID:38605034
Altered splicing machinery in lung carcinoids unveils NOVA1, PRPF8 and SRSF10 as novel candidates to understand tumor biology and expand biomarker discovery., PMID:38049848
Genetic Control of Alternative Splicing and its Distinct Role in Colorectal Cancer Mechanisms., PMID:37541527
PRPF8 controls alternative splicing of PIRH2 to modulate the p53 pathway and survival of human ESCs., PMID:37357506
TP53 and RB1 alterations characterize poor prognostic subgroups in pediatric acute myeloid leukemia., PMID:37102302
Retinitis pigmentosa-associated mutations in mouse Prpf8 cause misexpression of circRNAs and degeneration of cerebellar granule cells., PMID:37019475
Intermediate Uveitis in Retinitis Pigmentosa Associated with a Novel Homozygous Splice Site Mutation in PRPF8., PMID:36685344
Tumor-infiltrating lymphocytes mediate complete and durable remission in a patient with NY-ESO-1 expressing prostate cancer., PMID:36627144
Reovirus μ2 Protein Impairs Translation to Reduce U5 snRNP Protein Levels., PMID:36614170
PRPF8 increases the aggressiveness of hepatocellular carcinoma by regulating FAK/AKT pathway via fibronectin 1 splicing., PMID:36609600
U5 snRNP Core Proteins Are Key Components of the Defense Response against Viral Infection through Their Roles in Programmed Cell Death and Interferon Induction., PMID:36560714
Structural and functional investigation of the human snRNP assembly factor AAR2 in complex with the RNase H-like domain of PRPF8., PMID:36322420
Cilia regeneration requires an RNA splicing factor from the ciliary base., PMID:36180752
Integrated genomic sequencing in myeloid blast crisis chronic myeloid leukemia (MBC-CML), identified potentially important findings in the context of leukemogenesis model., PMID:35896598
Comprehensive analysis of spliceosome genes and their mutants across 27 cancer types in 9070 patients: clinically relevant outcomes in the context of 3P medicine., PMID:35719132
An epigenome-wide study of DNA methylation profiles and lung function among American Indians in the Strong Heart Study., PMID:35681244
Clinical exome sequencing for inherited retinal degenerations at a tertiary care center., PMID:35672425
Tissue-Specific Analysis of Alternative Splicing Events and Differential Isoform Expression in Large Yellow Croaker (Larimichthys crocea) After Cryptocaryon irritans Infection., PMID:35624193
Heterozygous variants in PRPF8 are associated with neurodevelopmental disorders., PMID:35543142
Reovirus μ2 protein modulates host cell alternative splicing by reducing protein levels of U5 snRNP core components., PMID:35489070
A human kinase yeast array for the identification of kinases modulating phosphorylation-dependent protein-protein interactions., PMID:35225431
The intrinsically disordered TSSC4 protein acts as a helicase inhibitor, placeholder and multi-interaction coordinator during snRNP assembly and recycling., PMID:35188580
Landscape of pathogenic variants in six pre-mRNA processing factor genes for retinitis pigmentosa based on large in-house data sets and database comparisons., PMID:35138024
The role of splicing factor PRPF8 in breast cancer., PMID:35124606
A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing., PMID:35033060
CryoEM of RUVBL1-RUVBL2-ZNHIT2, a complex that interacts with pre-mRNA-processing-splicing factor 8., PMID:34951455
Multifunctional RNA-binding proteins influence mRNA abundance and translational efficiency of distinct sets of target genes., PMID:34879078
LUBAC regulates ciliogenesis by promoting CP110 removal from the mother centriole., PMID:34813648
BRCA2 deficiency reveals that oxidative stress impairs RNaseH1 function to cripple mitochondrial DNA maintenance., PMID:34348152
The pioneer transcription factors Foxa1 and Foxa2 regulate alternative RNA splicing during thymocyte positive selection., PMID:34323272
PRPF19 regulates p53-dependent cellular senescence by modulating alternative splicing of MDM4 mRNA., PMID:34144037
For research use only. Not for human or drug use.
