Catalog No.
YHK58401
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Gly430-Glu774
Predicted molecular weight
40.51 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q6PCD5
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
RING finger and WD repeat domain-containing protein 3, RNF201, E3 ubiquitin-protein ligase RFWD3, RING finger protein 201, RFWD3
Elucidating the genetic mechanisms governing cytosine base editing outcomes through CRISPRi screens., PMID:40394064
Somatic gene mutations involved in DNA damage response/Fanconi anemia signaling are tissue- and cell-type specific in human solid tumors., PMID:39421870
Genetic Foundations of Nellore Traits: A Gene Prioritization and Functional Analyses of Genome-Wide Association Study Results., PMID:39336722
Heterozygous RPA2 variant as a novel genetic cause of telomere biology disorders., PMID:39231615
E3 ligases: a ubiquitous link between DNA repair, DNA replication and human disease., PMID:38985307
CK2-dependent degradation of CBX3 dictates replication fork stalling and PARP inhibitor sensitivity., PMID:38781342
RFWD3 modulates response to platinum chemotherapy and promotes cancer associated phenotypes in high grade serous ovarian cancer., PMID:38711848
MicroRNA-190b Targets RFWD3 in Estrogen Receptor-Positive Breast Cancer., PMID:38504674
SMARCAL1 ubiquitylation controls its association with RPA-coated ssDNA and promotes replication fork stability., PMID:38502677
Investigation of (Epi)genetic causes in syndromic short children born small for gestational age., PMID:37758162
RFWD3 promotes ZRANB3 recruitment to regulate the remodeling of stalled replication forks., PMID:37036693
Landscape of pathogenic mutations in premature ovarian insufficiency., PMID:36732629
RFWD3 acts as a tumor promotor in the development and progression of bladder cancer., PMID:36458571
RING finger and WD repeat domain 3 regulates proliferation and metastasis through the Wnt/β-catenin signalling pathways in hepatocellular carcinoma., PMID:36158256
RFWD3 and translesion DNA polymerases contribute to PCNA modification-dependent DNA damage tolerance., PMID:35905994
Systematic analysis on expression quantitative trait loci identifies a novel regulatory variant in ring finger and WD repeat domain 3 associated with prognosis of pancreatic cancer., PMID:35830250
Targeted Degradation of 53BP1 Using Ubiquitin Variant Induced Proximity., PMID:35454069
RFWD3 Participates in the Occurrence and Development of Colorectal Cancer via E2F1 Transcriptional Regulation of BIRC5., PMID:34712656
A comprehensive molecular study identified 12 complementation groups with 56 novel FANC gene variants in Indian Fanconi anemia subjects., PMID:34585473
The ubiquitin ligase RFWD3 is required for translesion DNA synthesis., PMID:33321094
The E3 ligase RFWD3 stabilizes ORC in a p53-dependent manner., PMID:33044890
Down-regulation of RFWD3 inhibits cancer cells proliferation and migration in gastric carcinoma., PMID:32902405
E3 ligase RFWD3 is a novel modulator of stalled fork stability in BRCA2-deficient cells., PMID:32391871
The Drosophila melanogaster Ortholog of RFWD3 Functions Independently of RAD51 During DNA Repair., PMID:31900333
Identification of an E3 ligase-encoding gene RFWD3 in non-small cell lung cancer., PMID:31571161
PCNA-mediated stabilization of E3 ligase RFWD3 at the replication fork is essential for DNA replication., PMID:30530694
Biallelic mutations in the ubiquitin ligase RFWD3 cause Fanconi anemia., PMID:28691929
A phosphorylation-and-ubiquitylation circuitry driving ATR activation and homologous recombination., PMID:28666352
RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination., PMID:28575658
RPA-Mediated Recruitment of the E3 Ligase RFWD3 Is Vital for Interstrand Crosslink Repair and Human Health., PMID:28575657
Genome-wide association study identifies multiple susceptibility loci for multiple myeloma., PMID:27363682
Variant in the RFWD3 gene associated with PATN1, a modifier of leopard complex spotting., PMID:26568529
RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled Replication Forks., PMID:26474068
Meta-analysis identifies four new loci associated with testicular germ cell tumor., PMID:23666239
RING finger and WD repeat domain 3 (RFWD3) associates with replication protein A (RPA) and facilitates RPA-mediated DNA damage response., PMID:21558276
E3 ligase RFWD3 participates in replication checkpoint control., PMID:21504906
RFWD3-Mdm2 ubiquitin ligase complex positively regulates p53 stability in response to DNA damage., PMID:20173098
For research use only. Not for human or drug use.
