Catalog No.
YVV22501
Expression system
E. coli
Species
Enterobacteria phage T4 (Bacteriophage T4)
Protein length
Ile2-Leu487
Predicted molecular weight
68.78 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P00970
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
DNA ligase, 6.5.1.1, Polydeoxyribonucleotide synthase [ATP], 30
Crystal structure of ATP-dependent DNA ligase from Rhizobium phage vB_RleM_P10VF., PMID:40365832
T7 RNA polymerase-mediated rolling circle transcription and the CRISPR-Cas13a cascade reaction for sensitive and specific detection of piRNA., PMID:39263843
Four Parallel Pathways in T4 Ligase-Catalyzed Repair of Nicked DNA with Diverse Bending Angles., PMID:38582512
Immune-check blocking combination multiple cytokines shown curative potential in mice tumor model., PMID:37199371
Programming CircLigase Catalysis for DNA Rings and Topologies., PMID:33382236
Cloning in Plasmid Vectors: Blunt-End Cloning., PMID:33139501
A general strategy for highly sensitive analysis of genetic biomarkers at single-base resolution with ligase-based isothermally exponential amplification., PMID:32113533
Direct and sensitive detection of circulating miRNA in human serum by ligase-mediated amplification., PMID:31514897
Target-catalyzed hairpin structure-mediated padlock cyclization for ultrasensitive rolling circle amplification., PMID:31357296
Ultrasensitive detection of lung cancer-associated miRNAs by multiple primer-mediated rolling circle amplification coupled with a graphene oxide fluorescence-based (MPRCA-GO) sensor., PMID:31123738
Primer remodeling amplification-activated multisite-catalytic hairpin assembly enabling the concurrent formation of Y-shaped DNA nanotorches for the fluorescence assay of ochratoxin A., PMID:30990481
Chimeric padlock and iLock probes for increased efficiency of targeted RNA detection., PMID:30309880
T4 DNA ligase structure reveals a prototypical ATP-dependent ligase with a unique mode of sliding clamp interaction., PMID:30169742
Thermal Stabilization of Biologics with Photoresponsive Hydrogels., PMID:29394044
Understanding DNA replication by the bacteriophage T4 replisome., PMID:28972188
[Synthesis of Circular DNA Templates with T4 RNA Ligase for Rolling Circle Amplification]., PMID:28900093
Multiplexed analysis of fixed tissue RNA using Ligation in situ Hybridization., PMID:28854731
Two-metal versus one-metal mechanisms of lysine adenylylation by ATP-dependent and NAD+-dependent polynucleotide ligases., PMID:28223499
In vitro circularization of RNA., PMID:27668458
A High Performance Platform Based on cDNA Display for Efficient Synthesis of Protein Fusions and Accelerated Directed Evolution., PMID:26812183
Efficient synthesis of stably adenylated DNA and RNA adapters for microRNA capture using T4 RNA ligase 1., PMID:26500066
Archaeal Nucleic Acid Ligases and Their Potential in Biotechnology., PMID:26494982
A simple and convenient sticky/blunt-end ligation method for fusion gene construction., PMID:25820211
[Application of repair enzymes to improve the quality of degraded DNA templates for PCR amplification]., PMID:25757334
A novel molecular beacon-based method for isothermal detection of sequence-specific DNA via T7 RNA polymerase-aided target regeneration., PMID:25613814
X-DNA origami-networked core-supported lipid stratum., PMID:25585044
Polynucleotide 3'-terminal phosphate modifications by RNA and DNA ligases., PMID:25324547
Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay., PMID:25063296
Structural studies of postentry restriction factors reveal antiparallel dimers that enable avid binding to the HIV-1 capsid lattice., PMID:24979782
Engineered DNA ligases with improved activities in vitro., PMID:23754529
Splint ligation of RNA with T4 DNA ligase., PMID:23065567
Structure-function analysis of Methanobacterium thermoautotrophicum RNA ligase - engineering a thermostable ATP independent enzyme., PMID:22809063
Model-guided ligation strategy for optimal assembly of DNA libraries., PMID:22543356
Fluorogenic substrates with single fluorophores for nucleic acid-modifying enzymes: design principles and new applications., PMID:22387388
Structural bias in T4 RNA ligase-mediated 3'-adapter ligation., PMID:22241775
Modulation of the processive abasic site lyase activity of a pyrimidine dimer glycosylase., PMID:21889915
RNA captor: a tool for RNA characterization., PMID:21533245
Phage T4-induced dTTP accretion bolsters a tRNase-based host defense., PMID:21481433
Detection of single-base mutation in RNA using T4 RNA ligase-based nick-joining or DNAzyme-based nick-generation., PMID:21453671
Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives., PMID:21129204
Amplification of unknown RNAs and RNA mixtures based on unique restriction enzyme cleavage in vitro., PMID:21106769
Optimization of enzymatic reaction conditions for generating representative pools of cDNA from small RNA., PMID:20921270
Generation of human PTH1R construct with FLAG epitope located internally: comparison of two-fragment assembly by using PCR overlap extension or ligase., PMID:19721821
Assembly and glycerol gradient isolation of yeast spliceosomes containing transcribed or synthetic U6 snRNA., PMID:18982283
Discovery of drug-like inhibitors of an essential RNA-editing ligase in Trypanosoma brucei., PMID:18981420
RNA ligases., PMID:18972386
Waste not, want not: a case for tRNA repair., PMID:18679433
Multiple segmental and selective isotope labeling of large RNA for NMR structural studies., PMID:18583361
The structure of an archaeal homodimeric ligase which has RNA circularization activity., PMID:18511537
Evaluation of NAD(+) -dependent DNA ligase of mycobacteria as a potential target for antibiotics., PMID:17548501
For research use only. Not for human or drug use.
