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Anti-KRT8/CK-8 Polyclonal Antibody (PHC17901)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P05787
Overview

Catalog No.

PHC17901

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human KRT8/CK-8 (Gln90-Gln236).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Type-II keratin Kb8,CYK8,Keratin-8,KRT8,Keratin,type II cytoskeletal 8,Cytokeratin-8,CK-8,K8

Concentration

0.4 mg/ml

Purification

Purified by antigen affinity column.

Accession

P05787

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with KRT8 / CK-8 antibody (PHC17901) at 0.4μg/ml.

    Lane 1: A549 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 53 kDa
    Observed MW: 53 kDa
  • Immunocytochemistry/ Immunofluorescence
    KRT8 / CK-8 in HaCat Cell Line.
    The HaCat cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with KRT8 / CK-8 (PHC17901) at 8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with KRT8/CK-8 antibody (PHC17901) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 20 kDa
    Observed MW: 20 kDa
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-KRT8/CK-8 Polyclonal Antibody [PHC17901]
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