Anti-Human IL17A Antibody (SAA0387) (FHH28820)

specific references (0) DATASHEET

Host species：Human

Isotype：IgG4, kappa

Applications：ELISA, FCM

Accession：Q16552

Overview

Catalog No.

FHH28820

Species reactivity

Human

Host species

Human

Isotype

IgG4, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Target

CTLA-8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17A, IL17A, CTLA8, IL-17, Interleukin-17A

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q16552

Applications

ELISA, FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.09% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0387

Data Image

Western Blot

Various lysates were subjected to SDS PAGE followed by western blot with Anti Human IL17A antibody [SAA0387] (FHH28820) at 1μg/ml.

Lane 1: Human IL17A transfected HEK293 cell lysate
Lane 2: Non-transfected HEK293 cell lysate

Second Ab: Goat Anti-Human IgG H&L Polyclonal antibody, HRP (PHB96431) at 0.1 μg/mL.

Predict MW: 18 kDa
Flow-cytometry

Flow-cytometry using anti-human IL17A antibody.IL17A Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL17A antibody monoclonal antibody (Catalog # FHH28820 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human IL17A antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL17A antibody monoclonal antibody (Catalog # FHH28820 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Document Download

Anti-Human IL17A Antibody (SAA0387).pdf

$ 198

Product specifications

50 μg 198 100 μg 328

Contact Information

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For research use only. Not for human or drug use.

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