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Anti-Human IL10 Antibody (SAA0378) (FHD50710)

Host species:Mouse
Isotype:IgG1, kappa
Applications:Blocking, ELISA, FCM
Accession:P22301
Overview

Catalog No.

FHD50710

Species reactivity

Human

Host species

Mouse

Isotype

IgG1, kappa

Clonality

Monoclonal

Conjugation

Unconjugated

Target

IL-10, IL10, Cytokine synthesis inhibitory factor, CSIF, Interleukin-10

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P22301

Applications

Blocking, ELISA, FCM

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.09% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA0378

Data Image
  • Flow-cytometry
    Flow-cytometry using anti-human IL10 antibody.IL10 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL10 antibody monoclonal antibody (Catalog # FHD50710 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with IL10 antibody (FHD50710) at 1 µg/ml.

    Lane 1: IL10 transfected HEK293 cell lysate
    Lane 2: Non-transfected HEK293 cell lysate

    Second Ab: Goat Anti-Mouse IgG H&L Polyclonal antibody, HRP (PMB96431) at 0.1 μg/mL.

    Predict MW: 31 kDa
    Observed MW: 31 kDa
References
Datasheet
$ 198
Product specifications
50 μg 198 100 μg 328

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human IL10 Antibody (SAA0378) [FHD50710]
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