InVivoMAb Anti-Human IL6 (Iv0022) (VHC15801)

specific references (0) DATASHEET

Host species：Human

Isotype：IgG1, kappa

Applications：ELISA, FCM, Neutralization

Accession：P05231

Overview

Catalog No.

VHC15801

Species reactivity

Human

Host species

Human

Isotype

IgG1, kappa

Clonality

Monoclonal

Target

Hybridoma growth factor, BSF-2, IFN-beta-2, CDF, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, Interferon beta-2, IFNB2, CTL differentiation factor

Concentration

2.28 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P05231

Applications

ELISA, FCM, Neutralization

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Iv0022

Data Image

Western Blot

Various lysates were subjected to SDS PAGE followed by western blot with Anti Human IL6 antibody [Iv0022] (VHC15801) at 1μg/ml.

Lane 1: Human IL6 transfected HEK293 cell lysate
Lane 2: Non-transfected HEK293 cell lysate

Second Ab: Goat Anti-Human IgG H&L Polyclonal antibody, HRP (PHB96431) at 0.1 μg/mL.

Predict MW: 25 kDa
Flow-cytometry

Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # VHC15801 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human IL6 antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # VHC15801 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PHB96441) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Document Download

InVivoMAb Anti-Human IL6 (Iv0022).pdf

$ 372

Product specifications

100 μg 372 1 mg 1486

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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