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Recombinant ASFV EP402R Protein, N-His

Catalog #:   YVV05901 Specific References (0) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: E. coli
Accession: A0A0A7W5A6
Protein length: Ser230-Ile360
Overview

Catalog No.

YVV05901

Expression system

E. coli

Species

African swine fever virus (ASFV)

Protein length

Ser230-Ile360

Predicted molecular weight

17.11 kDa

Nature

Recombinant

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

A0A0A7W5A6

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 5% Trehalose, 2% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

CD2 homolog, 5HL, CD2v, T-lymphocyte CD2 receptor-like protein, pEP402R, EP402R, EP402R CDS, ASFV-Georgia_4-082, ASFV_Kyiv_2016_131_00086

Note

For research use only.

Data Image
  • SDS-PAGE
    SDS-PAGE for Recombinant ASFV EP402R protein
  • Bioactivity
    b Specific binding antibodies against p72, p54, p30, CD2v, and p17 in sera were assessed on day 42 after the initial injection by indirect ELISA (n = 6).
    Data sourced from citation (PMID: 41577679)
  • Bioactivity
    b-f Serum IgG titers specific to p72,p54,p30,CD2v,and p17at indicated time points measured by indirect ELISA
    Data sourced from citation (PMID: 41577679)
  • ELISPot
    c Four mice of each group were euthanized on day 35. Splenocytes were isolated and subjected to in vitro stimulation with peptide pools (MTCE,p17,penton)(leftpanel) or proteins (p72, p54, p30, CD2v) (right panel). The induced IFN-γ cells were quantified by ELISpot and expressed as the number of spot-forming cells (SFCs) per million splenocytes.
    Data sourced from citation (PMID: 41577679)
  • ELISPot
    h Reactivities of PBMCs isolated from blood samples to stimulation of peptide pools (MTCE,p17,penton)(upperpanel) or proteins (p72,p54,p30,CD2v)(lowerpanel), measured by ELISpot and expressed as the number of IFN-γ secreting cells permillion PBMCs.
    Data sourced from citation (PMID: 41577679)
References
Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Recombinant ASFV EP402R Protein, N-His [YVV05901]
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