Buy Recombinant Human MUC16/CA125 Protein, C-His for ELISA

Recombinant Human MUC16/CA125 Protein, C-His

Catalog #: EHJ31001 Specific References (0) DATASHEET

Bioactivity for Recombinant Human MUC16/CA125 Protein, C-His ?>

Surface Plasmon Resonance for Recombinant Human MUC16/CA125 Protein, C-His ?>

Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Expression system: Mammalian Cells

Accession: Q8WXI7

Protein length: Met13360-Gln14347

Overview

Catalog No.

EHJ31001

Expression system

Mammalian Cells

Species

Homo sapiens (Human)

Protein length

Met13360-Gln14347

Predicted molecular weight

112.85 kDa

Nature

Recombinant

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

Q8WXI7

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Mucin-16, CA-125, Ovarian carcinoma antigen CA125, CA125, MUC-16, Ovarian cancer-related tumor marker CA125, MUC16

Note

For research use only.

Data Image

SDS-PAGE

SDS PAGE for Recombinant Human MUC16/CA125 Protein
Bioactivity

Detects Sofituzumab in indirect ELISAs.
Bioactivity

Detects Human MUC16/CA125 Antibody (SAA0160) in indirect ELISAs.
Bioactivity

Detects Human MUC16/CA125 Antibody (SAA0161) in indirect ELISAs.
Bioactivity

Detects Ubamatamab in indirect ELISAs.
Bioactivity

Detects Human MUC16 Antibody (AR9.6) in indirect ELISAs.
Bioactivity

c, Selectivity of the 1G8-S85AzF-functionalized SNAP-FET biosensor. Target protein concentration is 1 x 10−14 M, and interfering proteins were tested at 1 x 10−10 M, except for CA125 (1 x 10−5 U ml−1). Dots represent individual independent samples, and bars indicate mean ± standard deviation (n = 3).
Data sourced from citation (DOI: 10.1038/s44460-026-00040-4)
Bioactivity

(b) Normalized current response (ΔI/I0) of SNAP-FET functionalized with Orthogonal-NT2 compared with four random immobilization strategies (via carboxyl and amino) upon exposure to 100 U/mL CA125. Dots represent individual independent samples, and bars indicate mean ± standard deviation (n = 3).(c) Normalized current response ΔI/I0 of NT2-Q13AzK-modified SNAPFET and bare In2O3 at different CA125 concentrations. Error bars indicate mean ± standard deviation (n = 3).
Data sourced from citation (DOI: 10.1038/s44460-026-00040-4)
Bioactivity

(c) Normalized signal response (ΔI/I0) measured at VGS= 215 mV (Ag/AgCl side gate) and VGS= 200 mV (Ag/AgCl external gate) of In2O3 FET biosensors. The biosensors were tested in PBS upon exposure to HE4 (1x 10-14 M) and CA125 (3.75 x 10-2 U/mL). Dots represent individual independent samples, and bars indicate mean ± standard deviation (n = 8). Statistical significance was assessed using a two-sided unpaired t-test. No significant difference was observed between the two gating configurations for either biomarker (HE4, P = 0.7718; CA125, P = 0.0808); ns indicates not significant.
Data sourced from citation (DOI: 10.1038/s44460-026-00040-4)
Bioactivity

Transfer characteristic curves of (a) NT2-Q13AzK functionalized SNAP-FET and (b) bare In2O3 FET in response to gradient concentrations of CA125. The functionalized FET exhibits clear concentration dependent shifts upon CA125 addition, whereas the bare In2O3 FET shows negligible changes, representing baseline noise.
Data sourced from citation (DOI: 10.1038/s44460-026-00040-4)
Surface Plasmon Resonance

Surface plasmon resonance (SPR) analysis of wild-type nanobody NT2 binding to CA125. (a) Real-time sensorgrams of NT2 binding at concentrations of 0.78125, 3.125, 6.25, 12.5, and 25 nM. Responses were recorded for 100 s association and 180 s dissociation phases in PBS buffer (pH 7.4, 25℃). (b) Equilibrium binding analysis: Response units at steady state (t = 100 s) plotted against NT2 concentration. Data fitted to a 1:1 Langmuir binding model, yielding a dissociation constant KD = 3.417x10-9 M.
Data sourced from citation (DOI: 10.1038/s44460-026-00040-4)
Bioactivity

Specificity assessment of NT2-Q13AzK-functionalized SNAP-FET against 1x10-10 M interferents, with CA125 concentration of 3.75x10-2 U/mL. Dots represent individual independent samples, and bars indicate mean ± standard deviation (n = 3).
Data sourced from citation (DOI: 10.1038/s44460-026-00040-4)

References

Datasheet

Document Download

Recombinant Human MUC16/CA125 Protein, C-His.pdf

$ 480

Product specifications

100 μg 480 1 mg 2880

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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