Affiliations
- Department of Orthopedics, The First Affiliated Hospital of Soochow University, No.188 Shizi Street, Suzhou, 215000, Jiangsu, China.
- Department of Orthopedics, The Third Affiliated Hospital of Shanghai University, The Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou, 325000, China.
- Department of Orthopedics, The First Affiliated Hospital of Soochow University, No.188 Shizi Street, Suzhou, 215000, Jiangsu, China. xuyaozeng@163.com.
PMID: 37691099 PMCID: PMC10494329 DOI: 10.1186/s13018-023-04147-z
Abstract
Background: Osteoarthritis (OA) is a common geriatric arthritis marked by inflammation and cartilage degradation. This study investigates the role of lncRNA NEAT1 in OA pathogenesis through the miR-374b-5p/PGAP1 pathway.
Methods: OA cell models were built in LPS-induced human chondrocytes. Gene expression, cell cycle, apoptosis, and cytokine secretion were assessed. OA rat models were established for pathological analysis.
Results: NEAT1 was downregulated, while miR-374b-5p was upregulated in OA patients and models. NEAT1 targeted miR-374b-5p to regulate PGAP1 expression, influencing apoptosis, cell cycle, and inflammatory cytokine secretion in chondrocytes. Upregulated miR-374b-5p diminished PGAP1's protective effects.
Conclusions: The NEAT1/miR-374b-5p/PGAP1 axis may be a therapeutic target for OA treatment.
Keywords
Osteoarthritis, NEAT1, miR-374b-5p, PGAP1, Apoptosis, Inflammation, Cell cycle
